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There are several things to understand here.

Does EDuke32 have an Alsa MIDI output?
You must configure it, if possible, to have a MIDI output port. I don't know if that application creates MIDI output ports.

In Qtractor, in the connections window, go to the MIDI tab. Connect the EDuke32 MIDI output (if it exists) to the Master MIDI input. And then, as indicated in the Qtractor MIXER, activate the MIDI MASTER BUS monitor (not to be confused with the audio Master Bus) where the plugin is located.

PS
I've reviewed the links you attached and the EDuke32 documentation very briefly.

Maybe I'm wrong but...
Nowhere do I see that EDuke32 has MIDI output ports.
It's like trying to connect a Casio VL-1 to Qtractor to use it as a MIDI controller.

https://en.wikipedia.org/wiki/Casio_VL-1

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